propidium iodide pi  (Elabscience Biotechnology)

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A fluffy nanofiber scaffold-based microenvironment enables a simplified, biology-centric scale-up strategy for mesenchymal stem/stromal cell culture

doi: 10.3389/fbioe.2026.1808384

Figure Lengend Snippet: Dynamic suspension culture using mixed of chitosan and chitin nanofiber scaffolds. Comparison of agitation conditions in the presence of chitosan/chitin (80:20) using a 30-mL bioreactor. (A) Specific growth rate ( μ ) during 24–96 h. Data are mean ± s.d.; no significant difference was detected (Tukey’s test, n = 3 per culture). (B) Distribution of aggregate projected area at t = 96 h. Comparison of scaffold conditions at 50 rpm in a 30-mL bioreactor. (C) Growth profiles under without scaffolds (open triangle, △), chitin only (open circle, ○), and chitosan/chitin (80:20) (closed circle, ●). Data are mean ± s.d. ( n = 5 per culture). (D) Specific growth rate ( μ ) during 24–120 h. Data are mean ± s.d. *: p < 0.01 (Tukey’s test, n = 5 per culture). (E) Representative Live/Dead staining images: live cells (Calcein-AM, green) and dead cells (Propidium iodide, PI, red). Scale bar: 500 μm. (F) Distribution of aggregate projected area over 0.3 × 10 5 μm 2 at t = 120 h. (G) Aggregate number over 0.3 × 10 5 μm 2 at t = 120 h. Data are mean ± s.d. *: p < 0.01 (Student’s t-test, n = 5 per culture). (H) Confocal fluorescence images of aggregate sections cultured with FITC-labelled chitin (green) only or with California Red-labelled chitosan (red) at t = 72 and 120 h using a 30-mL bioreactor at 50 rpm. Blue shows nuclei (DAPI). Scale bar: 100 μm.

Article Snippet: Sampled cells were rinsed with D-PBS(−) and stained with 1.0 μg/mL Calcein-AM (C326; DOJINDO) and 2.0 μg/mL Propidium iodide (PI) (P378; DOJINDO) in a multi-well plate for 15 min at 37 °C.

Techniques: Suspension, Comparison, Staining, Fluorescence, Cell Culture

Journal: Cell Death Discovery

Article Title: WWP1 gain-of-function drives developmental anoikis through TGFβ pathway during neurodevelopment

doi: 10.1038/s41420-026-02977-4

Figure Lengend Snippet: A Scheme of WWP1 variant-expressing cell line establishment and downstream analysis. The image is created with Biorender.com. B Representative images of dead cell population in WWP1 variants-expressing HeLa cell lines treated with gefitinib. Red, propidium iodide (PI). Scale bar, 200 μm. C Quantification of the PI + counts in HeLa cell lines treated with gefitinib. n = 3 for each condition. Two-way ANOVA with Tukey’s post hoc test. * p < 0.05; ** p < 0.01. D Functional GO enrichment analysis of upregulated and downregulated DEGs between WWP1 E798V and control cell lines. The bar color indicates the cluster categories of the GO term. BP, biological process; CC, cellular component. E Differential PROGENy pathway activity scores of WWP1 E798V relative to control. The pathway with the lowest value, TGFβ, is indicated by a deep blue color. F Gene set enrichment plots for TGFβ signaling and epithelial-mesenchymal transition. NES, normalized enrichment score. G Western blot analysis of phospho-SMAD2 and SMAD2 in WWP1 variant-expressing HeLa cell lines treated with vehicle or hTGFβ1 (10 ng/mL). ACTIN is used as the loading control. H Quantification of normalized pSMAD2 to SMAD2 protein expression ratio in WWP1 variant-expressing HeLa cell lines. n = 5. Kruskal-Wallis test with Dunn’s post hoc test. * p < 0.05; ns not significant. Bar graphs indicate mean ± SEM.

Article Snippet: Non-cytotoxic dosing conditions were determined by the cell viability using Propidium Iodide/RNase staining solution (Cell Signaling Technology, #4087).

Techniques: Variant Assay, Expressing, Functional Assay, Control, Activity Assay, Western Blot